A Batagur Baska turtle, a rare species of the animal, has laid 23 more eggs at the Karamjal Wildlife Breeding Centre on the eastern side of the Sundarbans.
The eggs were laid on Wednesday evening, adding to the 27 eggs another turtle of the same species laid on February 28.
The 50 eggs have been kept in the sand following the natural process.
They are expected to hatch in 65-67 days, according to the officials.
Azad Kabir, the wildlife official in charge of the Karamjal Wildlife Breeding Centre, said Batagur Baska, a critically endangered species of riverine turtle, was found in six waterbodies in Noakhali and Barisal in 2008.
Eight turtles were found at the time - four male and four female.
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In the same year, the Forest Department moved the turtles to Gazipur, where the females laid 94 eggs.
In 2014, the originally found eight turtles and the 94 others were all moved to Karamjal Wildlife Breeding Centre.
In 2017, two turtles laid 63 eggs of which 57 hatched. In 2018, two turtles laid 46 eggs, of which 21 hatched.
In 2019, one turtle laid 32 eggs and all of them hatched; and in 2020, one turtle laid 35 eggs, of which 34 hatched.
Two Batagur Baska turtles were released in the rivers of the Sundarbans in 2017, five in 2018 and five more in 2019 from the wildlife breeding centre, Azad Kabir said.