The Brazilian football federation (CBF) announced on Sunday injured full-back Wesley will be replaced in the World Cup squad by midfielder Ederson.
Wesley suffered the injury in Saturday's 2-1 friendly win over Egypt before leaving the field in tears.
Atalanta's 26-year-old Ederson made the last of his three international appearances in March 2025.
"The Brazilian Football Confederation announces that the player Wesley was re-evaluated this Sunday by the medical staff of the Brazilian National Team and underwent imaging tests," the CBF said.
"The MRI revealed a muscle injury in the adductor muscle of his left thigh.
"In light of the diagnosis, the CBF announces the call-up of the player Ederson, who will join the squad this Monday in the United States," the governing body added.
Five-time World Cup winners Brazil start their campaign on Saturday against Morocco before facing Haiti and Scotland in Group C.
