Alessandro Del Piero, Shinji Ono and Emile Heskey will not play for Australia's A-League All Stars against Manchester United in Sydney later this month, the Football Federation Australia said on Friday.
The three high-profile overseas stars polled strongly in the online fan voting to be included in the line-up for the July 20 pre-season friendly against the English Premier League champions.
FFA chief executive David Gallop said the players' personal circumstances meant it was difficult for them to commit to the match at the Sydney Olympic stadium.
"Alessandro, Shinji and Emile have turned on great performances and brought a huge global and national profile to the A-League, so naturally the fans wanted to see them face Manchester United," Gallop said in a statement.
"But we respect the fact that their personal circumstances at this time mean the All Stars game arrives at a difficult period for them to manage a one-off appearance."
Gallop said factors including injuries, retirements and transfers during the off-season were always going to influence the final A-League All Stars selection.
German Thomas Broich and former Australian international Brett Emerton are among the players to have so far committed to playing against Manchester United.