The 36-year-old Serena was handed a code violation for coaching, a penalty point for racquet abuse and a game penalty for calling umpire Carlos Ramos a "liar and a thief" and insisting "you owe me an apology"
Serena Williams insisted she was not cheating in the US Open final on Saturday before accusing the sport which has made her a global icon and multi-millionaire of sexism.
Naomi Osaka won the final 6-2, 6-4 to become Japan's first ever Grand Slam singles champion and delay Williams's bid for a record-equalling 24th major title.
However, the final was overshadowed by the American's angry and tear-filled tirade in the second set.
It has already been dubbed 'The Mother of all Meltdowns' by the New York Daily Post.
The 36-year-old was handed a code violation for coaching, a penalty point for racquet abuse and a game penalty for calling umpire Carlos Ramos a "liar and a thief" and insisting "you owe me an apology".
US Open 2018: Serena Williams accuses umpire of sexism after meltdown in final https://t.co/T6XPondkV5— BBC News (UK) (@BBCNews) September 9, 2018
"He alleged that I was cheating, and I wasn't cheating," Williams told reporters later.
"I don't use on-court coaching (where it's allowed at WTA tour events).
"One thing I love about tennis is being out there. It's the one time I don't want to hear anyone tell me anything. You have to figure out. You have to problem-solve."
"You owe me an apology!"— ESPN (@espn) September 8, 2018
Serena was fired up with the official in the final set of the US Open final. pic.twitter.com/r6RSbrirnV
Williams said that her coach Patrick Mouratoglou had not been coaching her even though the Frenchman told ESPN that he had and that all coaches do it.
"I just texted Patrick, like, What is he talking about? Because we don't have signals. We have never discussed signals," said Williams.
Williams said the incident strengthened her belief that women players are treated differently to their male counterparts in the sport.
"I've seen other men call other umpires several things. I'm here fighting for women's rights and for women's equality," she claimed.
Everyone should listen to this from Serena Williams. pic.twitter.com/TF03dhpq2P— Cameron Cox (@CamCox12) September 8, 2018
"For me to say 'thief' and for him to take a game, it made me feel like it was a sexist remark.
"He's never taken a game from a man because they said 'thief'. For me it blows my mind. But I'm going to continue to fight for women."
Williams made reference to the incident last week when French player Alize Cornet was warned for removing her shirt on court during a heatwave.
Must read Sally Jenkins: “Williams abused her racket, but (umpire) Ramos did something far uglier: He abused his authority.” https://t.co/nAlSkt0k9q— Philip Rucker (@PhilipRucker) September 9, 2018
Cornet was accused of "unsportsmanlike behaviour" before tournament chiefs apologised, admitting the umpire made the wrong decision.
"Cornet should be able to take off her shirt without getting a fine. This is outrageous," said Williams warming to her theme.
"I just feel like the fact that I have to go through this is just an example for the next person that has emotions, and that want to express themselves, and want to be a strong woman.
"You know my character... this is not fair, to lose a game like that, there are men out there, this has happened too many times... there are men who have said a lot worse" @serenawilliams @usopen pic.twitter.com/hQeU0zmPlk— Neal Collins (@nealcol) September 9, 2018
"They're going to be allowed to do that because of today. Maybe it didn't work out for me, but it's going to work out for the next person."
Williams left the media interview room to applause.
Meanwhile, Mouratoglou took to Twitter to also take aim at Ramos.
"The star of the show has been once again the chair umpire," he wrote.
In ESPN interview with Pam Shriver, Patrick Mouratoglou admits that "I was coaching," and adds that Osaka's coach, Sascha Bajin, was coaching too—along with 100% of coaches on tour. #USOpen— TENNIS.com (@Tennis) September 8, 2018
"Should they be allowed have an influence on the result of a match? When do we decide that this should never happen again?"