Ballon d'Or winner Karim Benzema has been ruled out of the World Cup with a left thigh injury Saturday, dealing a serious blow to defending champions France's hopes of retaining the trophy.
The 34-year old's injury - suffered in training earlier Saturday - would require "three weeks of convalescence", the French Federation said in a statement.
The Real Madrid striker has been struggling with a thigh problem since October and has played less than half an hour of football in his club's last six games.
This was the first time Benzema had taken part in full training since Didier Deschamps' squad met up at their French headquarters of Clairefontaine last Monday.
Benzema, who last month won the Ballon d'Or, has not played an entire match since October 19.
He and Raphael Varane trained apart from the rest of the squad Thursday during a session open to the media at the home stadium of Qatari champions Al Sadd.
Manchester United center-back Varane has not played a game since coming off in tears against Chelsea on October 22.
Under Fifa rules Deschamps can call up a replacement for an injured player until Monday, on the eve of their first game against Australia.
This is a major headache for Deschamps, already deprived of the services of midfielders Paul Pogba and N'Golo Kante, No 2 goalkeeper Mike Maignan, defender Presnel Kimpembe and forward Christopher Nkunku.